Introduction
to Redistribution® Technology
Redistribution® technology is a cell based
assay technology that uses protein translocation as a readout
for the activity of cellular signalling pathways.
Redistribution®
assays are high throughput, high content cell based assays
primarily used for profiling of lead series, primary screening
of compound libraries, and as readouts for gene silencing
studies using siRNA.
Target
classes that are amenable to Redistribution®
technology:
Redistribution® technology is based on:
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Labelling targets with fluorescent proteins (e.g. Green
Fluorescent Protein, GFP)
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Generation of stably transfected assay cell lines
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Measurement of protein translocation
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Image analysis and quantification of cellular responses.
An
example of Redistribution® assay. A MAPK/ERK
pathway assay reporting on the activity of the MEK inhibitor
U0126 (the translocating target is ERF - an ERK
substrate).
Redistribution®
is BioImage's broadly enabling, proprietary technology that
covers any genetically encoded protein fusion to a luminophore
(e.g. GFP), with subsequent monitoring of the protein's
translocation within a cell as the primary readout for drug
discovery assays.
-
The trademarks BioImage and Redistribution®
used herein are owned by Fisher BioImage ApS, Denmark; BioImage is part of Thermo Fisher Scientific.
-
The practice of translocation assays as described herein
may violate BioImage patents US 6,518,021; EP 0,986,753B1;
US 6,172,188; EP 0,851,874, as well as other pending and
granted patents.
-
The material contained herein is subject to copyright,
but may be reproduced if Fisher BioImage ApS is properly acknowledged
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